These resources have been reviewed and selected by STEM Learning’s team of education specialists for factual accuracy and relevance to teaching STEM subjects in UK schools.

DNA Libraries: Bacterial Artificial Chromosome (BAC)

Produced by the Wellcome Trust Sanger Institute, this resource contains an overview of the techniques involved in making a BAC (Bacterial Artificial Chromosome) library. It consists of an animation that covers the key stages in the process:

* Extracting DNA from white blood cells and use of restriction enzymes to "cut up" DNA fragments;
* Gel electrophoresis to separate and select DNA fragments by size;
* Creating BAC clones using recombinant DNA techniques;
* Inserting DNA fragments into vectors and transferring in to host bacterial cells;
* Selection of the clones for the library.

This animation can be used to support teaching or recombinant DNA techniques in the classroom.

To play the animation, first download the file and save it to your computer, where it will run as a Flash animation.

Show health and safety information

Please be aware that resources have been published on the website in the form that they were originally supplied. This means that procedures reflect general practice and standards applicable at the time resources were produced and cannot be assumed to be acceptable today. Website users are fully responsible for ensuring that any activity, including practical work, which they carry out is in accordance with current regulations related to health and safety and that an appropriate risk assessment has been carried out.

Information on the permitted use of this resource is covered by the Category Three Content section in STEM Learning’s Terms and conditions.