LC-MS for the chromatographer HC290

Gain critical knowledge of liquid chromatography (LC) and mass spectrometry (MS) applications and instrumentation.


Summary

This exciting and interactive course contains critical knowledge of Liquid Chromatography (LC) and Mass Spectrometry (MS) applications and instrumentation. You will learn the absolute essentials of the technique and, using the unique multi-media examples and tutorial exercises, you will implement new knowledge and ideas immediately. The Atmospheric Pressure Interface (API) is the core element to the course with the principles of operation, limitations and applicability fully explored. The course covers ion suppression, the use of electrospray or APCI and MS-MS data acquisition modes. Optimisation of interface and mass filter settings and how to best utilise reduced dimension LC to speed up sample throughput will be discussed. All popular interface types and mass analysing equipment (quadrupole, TOF, ion trap, etc.) are comprehensively covered.

This course is recommended for users of LC-MS and LC-MS-MS equipment, or anyone interested in learning the analytical capabilities afforded by this type of instrument. No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial.

Outcomes

What you will learn:

  • how modern API designs work and which one best fits your LC and application requirements
  • how ions are produced and how to optimise LC mobile phase and interface parameters to achieve highest sensitivity, accuracy and reproducibility
  • the anatomy and working principles of quadrupoles, ion traps, magnetic sector, time-of-flight detectors and more
  • the information that can be acquired in MS, MS-MS and MSn modes, and how to optimise instrument settings for high data quality

Notes

We can set this up as a bespoke course to be run at your premises. Contact us at heated@stem.org.uk for more information.

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