Cauliflower Cloning

Overall rating
5
5
Based on 2 reviews
5
2
4
0
3
0
2
0
1
0
5
Rate this resource

This video introduces a reliable technique to demonstrate tissue culture and totipotency, and shows how best to use this protocol with your students.

Unlike animal cells, many plant cells are totipotent, meaning that each cell has the capacity to regenerate the entire plant. Micropropagation is the regeneration of whole plants from small pieces of plant material. In this case students use a tiny piece of a cauliflower, in place of the endangered species that the protocol was designed for. Since all the explants come from the same cauliflower, the new plants will be clones of each other.

The technique is based on a protocol developed by plant scientists at the Royal Botanic Gardens, Kew, and is used in their conservation programmes, allowing critically endangered plant material to be cloned in the field, rather than waiting until a 'clean lab' can be found to rescue plant material.

 

Show health and safety information

Please be aware that resources have been published on the website in the form that they were originally supplied. This means that procedures reflect general practice and standards applicable at the time resources were produced and cannot be assumed to be acceptable today. Website users are fully responsible for ensuring that any activity, including practical work, which they carry out is in accordance with current regulations related to health and safety and that an appropriate risk assessment has been carried out.

Published by

Actions

Share this resource

Lists that tag this content

Biology, POSTED BY