DNA Sequencing

From the Wellcome Trust, these animations show three different methods for the sequencing of DNA.

The Sanger method, developed in 1975, illustrates an early way of determining a DNA sequence. Fluorescent bases are incorporated into fragments of DNA that are made from the sample being sequenced. Analysis of the fragments enables the sequence of bases in the original strand of DNA to be determined. Although it is slow in comparison to more modern methods, its development was a major breakthrough in DNA sequencing.

Developed in 2005 and 2006, the 454 and Illumina methods allow for greater automation and speed of sequencing. Both methods use fragments of DNA attached to a solid substrate. This allows DNA sequences to be determined much more quickly than the Sanger method. The Illumina method takes just half a day to read one gigabase and can run multiple samples simultaneously.

The animations contain quite a high level of detail and are suited to students following post-16 biology courses.

Show health and safety information

Please be aware that resources have been published on the website in the form that they were originally supplied. This means that procedures reflect general practice and standards applicable at the time resources were produced and cannot be assumed to be acceptable today. Website users are fully responsible for ensuring that any activity, including practical work, which they carry out is in accordance with current regulations related to health and safety and that an appropriate risk assessment has been carried out.

Downloads

You might also like

Resource
Resource
Resource
Resource

Published by

Actions

Share this resource

Lists that tag this content

Comments