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Determining Dry Weight

This experiment is a continuation of Determining Doubling Time. Students create a standard curve of absorbance against yeast concentration, and use this to determine the concentration of yeast produced over time.

The standard curve (dry mass, grams per litre) is generated from known concentrations of dried yeast in sterile broth. Commercially available dried yeast is used to produce the yeast suspensions. From the graph students can determine the concentration, in grams per litre, of yeast produced over time using the absorbances they obtained in the previous experiment.

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