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Gel Electrophoresis of DNA

Produced by Science & Plants for Schools (SAPS), this activity enables students to look at the gel electrophoresis of DNA treated with restriction enzymes. It will help to develop a knowledge and understanding of the cutting of DNA with restriction enzymes.

In the experiment, a batch of DNA is digested by two different restriction enzymes. The fragments are separated by gel electrophoresis and the difference in patterns analysed.

The investigation is designed for students following a Scottish Highers course but it is equally useful for other post-16 courses in biology. It could also be adapted for younger secondary school students.

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