Microscopy of root tip mitosis

5
0
4
0
3
0
2
0
1
0
0
Rate this resource

In plants, cell division by mitosis takes place in specific tissues, called meristems, that are found at the growing root and shoot tips and in the cambium between the xylem and phloem of the vascular bundle. The cells in the meristems are undifferentiated. In this practical, students will prepare and observe dividing cells from the meristems of actively growing garlic root tips.

In this practical protocol, toluidine blue is used to stain the DNA in the meristem cells, instead of the more traditionally-used acetic orcein. This is because:

  • Toluidine blue has been found to produce a level of contrast within the cells that allows chromosomes to be clearly seen

  • The protocol developed here, using toluidine blue, is a simpler process than protocols involving acetic orcein

  • The use of toluidine blue in a school laboratory is less hazardous than the use of acetic orcein

Show health and safety information

Please be aware that resources have been published on the website in the form that they were originally supplied. This means that procedures reflect general practice and standards applicable at the time resources were produced and cannot be assumed to be acceptable today. Website users are fully responsible for ensuring that any activity, including practical work, which they carry out is in accordance with current regulations related to health and safety and that an appropriate risk assessment has been carried out.

Published by

Actions

Share this resource