Electrophoresis gel with annotation

Di-deoxy sequencing is a way to determine the order of bases (A,C,G and T) in a piece of DNA. A set of 4 lanes (one for each base) is needed to sequence any given piece of DNA. Wherever a black
band appears in a particular lane that base is present. By reading off the order of bands across the four lanes the sequence of bases can be worked out. This is illustrated for the section of the
sequence marked out by the lines.

Credit: Guy Tear, Wellcome Images

This resource is part of the Post 16 genetics and genomics collection of resources.

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