Practical HPLC method development HC296 / A03

Start date
7 Jun 16
2 Days
Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA View on Google Map


For the experienced chromatographer, this course provides a step-by-step approach to method development. The course includes all the crucial aspects of method development, including information gathering, mode of chromatography, development of mobile phase systems, column choice, principles of ionisation/suppression, optimisation of important chromatographic parameters (R, alpha, k' and N), isocratic and gradient operation and detector choice and optimisation. Each aspect is discussed in detail with relevant examples used to demonstrate theoretical principles. Chromatographic modelling software-based exercises are included to give further understanding, and practical laboratory sessions are included to make an all-encompassing two day course.

This course is for anyone wishing to have the most comprehensive grounding in instrumental analysis techniques or those wishing to increase their knowledge and practical ability. Some experience of operating HPLC equipment and analysing HPLC data is required in order to fully participate in this course.


What you will learn:

  • setting method development objectives
  • effect of column parameters
  • developing methods for neutral samples
  • developing a separation for a mixture of neutral, acidic and basic compounds
  • using a scouting gradient
  • optimisation strategies
  • quantitation and system characterisation


Practical HPLC method development07 June 201609:00-17:00Glasgow Caledonian University


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heated memberActivity fee£680.00 +VAT
heated non-memberActivity fee£744.00 +VAT

Participants from outside the UK will be charged the course fee and an additional £120 per day plus VAT.

Outside the UK? Fee information

Additional information

For courses at the National STEM Learning Centre in York, fees include meals and accommodation for the duration of the course. 


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