Practical HPLC method development HC296 / A03
For the experienced chromatographer, this course provides a step-by-step approach to method development. The course includes all the crucial aspects of method development, including information gathering, mode of chromatography, development of mobile phase systems, column choice, principles of ionisation/suppression, optimisation of important chromatographic parameters (R, alpha, k' and N), isocratic and gradient operation and detector choice and optimisation. Each aspect is discussed in detail with relevant examples used to demonstrate theoretical principles. Chromatographic modelling software-based exercises are included to give further understanding, and practical laboratory sessions are included to make an all-encompassing two day course.
This course is for anyone wishing to have the most comprehensive grounding in instrumental analysis techniques or those wishing to increase their knowledge and practical ability. Some experience of operating HPLC equipment and analysing HPLC data is required in order to fully participate in this course.
What you will learn:
- setting method development objectives
- effect of column parameters
- developing methods for neutral samples
- developing a separation for a mixture of neutral, acidic and basic compounds
- using a scouting gradient
- optimisation strategies
- quantitation and system characterisation
|Practical HPLC method development||07 June 2016||09:00-17:00||Glasgow Caledonian University|
All of our courses will contribute to your evidence base for professional registration. Just check against your competency measures to make sure you use our training and development in your evidence records.
|heated member||Activity fee£680.00 +VAT|
|heated non-member||Activity fee£744.00 +VAT|
Participants from outside the UK will be charged the course fee and an additional £120 per day plus VAT.
For courses at the National STEM Learning Centre in York, fees include meals and accommodation for the duration of the course.
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