HPLC troubleshooting and maintenance HC289 / F02

Start date
2 Mar 16
1 Day
King's College London, Kings College London, Strand, London WC2R 2LS View on Google Map


This one day course introduces the fundamentally important concepts associated with troubleshooting HPLC analysis including hardware basics, separation issues, peaks shape and baseline issues and more.

All components of the HPLC system and associated problems are systematically studied. The use of chromatograms as diagnostic tools is explored in depth, including baselines, peak shape and retention time inconsistencies, spurious peaks, etc.

Suitable as a refresher for the more experienced analytical chemist or as an invaluable introduction to the technique for those with limited experience, this course provides an invaluable insight into HPLC principles and practice.
This course is designed for those relatively new to HPLC, and ideally will follow on from attendance on the Fundamental HPLC training course.

No previous knowledge of chromatography is necessary, but basic experience of chemistry is beneficial. Some experience of operating HPLC equipment and analysing HPLC data is required in order to fully participate in this course.


What you will learn:
- how to optimise instrument and method parameters to obtain high quality spectral data
- ionisation and fragmentation mechanisms in GC-MS
- logical evolution of an interpretation toolkit with a multitude of practice spectra with worked solution on which to practise new skills
- the fundamental spectral interpretation toolkit, and how to use it when confronted with the mass spectrum of an unknown


HPLC troubleshooting and maintenance02 March 201609:00-17:00King's College London


All prices exclude VAT which will be added at the standard rate.

Cancellation notice must be received in writing at least 14 days before the course delivery date. A charge of 20% of the course fee will be made to cover administration and manual preparation costs. This is in addition to the usual HEaTED Terms & Conditions agreed to at the time of submitting a booking.

All of our courses will contribute to your evidence base for professional registration https://www.heated.ac.uk/professional-registration/ Just check against your competency measures to make sure you use our training and development in your evidence records.

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heated memberActivity fee£178.00 +VAT
heated non-memberActivity fee£194.00 +VAT

Participants from outside the UK will be charged the course fee and an additional £120 per day plus VAT.

Outside the UK? Fee information

Additional information

For courses at the National STEM Learning Centre in York, fees include meals and accommodation for the duration of the course. 


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