Fundamental HPLC HC284 / F02

Start date
1 Mar 16
1 Day
King's College London, Kings College London, Strand, London WC2R 2LS View on Google Map


This course introduces the fundamentally important concepts associated with High Performance Liquid Chromatography (HPLC) analysis including hardware basics, modes of analysis, basic troubleshooting, column chemistry, important parameters of the mobile phase such as amount and type of organic solvents, pH, buffer strength and type, and much more.

Suitable as a refresher for the more experienced analytical chemist or as an invaluable introduction to the technique for those with limited experience, this course provides a priceless insight into HPLC principles and practice. This course is not instrument-specific.

This course is recommended for users of LC in any of its modes (NP, RP, HILIC, IEX, SEC…) or anyone interested in gaining a deeper understanding of the chromatographic separation of compounds amenable to analysis by LC.
No previous experience of chromatography is necessary, but a basic knowledge of chemistry is beneficial.


What you will learn:
- how the chromatographic process takes place
- the essential theory behind chromatographic separations
- the architecture and working principles of a modern HPLC system
- the information contained in a chromatogram and the basic chromatographic parameters
- the essential principles of sample preparation, mobile phases, buffers and eluent additives for HPLC


Fundamental HPLC01 March 201609:00-17:00King's College London


All prices exclude VAT which will be added at the standard rate.

Cancellation notice must be received in writing at least 14 days before the course delivery date. A charge of 20% of the course fee will be made to cover administration and manual preparation costs. This is in addition to the usual HEaTED Terms & Conditions agreed to at the time of submitting a booking.

All of our courses will contribute to your evidence base for professional registration Just check against your competency measures to make sure you use our training and development in your evidence records.

Share with your CPD booker


heated memberActivity fee£178.00 +VAT
heated non-memberActivity fee£194.00 +VAT

Participants from outside the UK will be charged the course fee and an additional £120 per day plus VAT.

Outside the UK? Fee information

Additional information

For courses at the National STEM Learning Centre in York, fees include meals and accommodation for the duration of the course. 


Interested in this course?

Sign up to receive our emails to hear about the latest CPD available