The effect of phosphate on the enzyme phosphatase

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Produced by Science & Plants for Schools (SAPS), this investigation looks at end-product inhibition of the enzyme phosphatase. 

The investigation is designed for students following a Scottish Highers course but it is equally useful for other post-16 courses in biology. 

This investigation involves an acid phosphatase extracted from germinating mung beans (beansprouts). A simple aqueous extract is used as the enzyme solution. An artificial substrate, phenolphthalein phosphate is used. The phosphate, being an end-product, also inhibits the enzyme. This experiment will demonstrate that the higher the concentration of phosphate present, the greater the inhibition of the enzyme.

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