The Optical Microscope in Biology

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Produced in 1976 by the Institute of Biology as part of its Studies in Biology series, this booklet introduces the use of the optical microscope in biology. The use of contrast-enhancing techniques allows the detailed structural changes of living cells to be studied, whilst fluorescent markers allow dynamic processes to be observed. Methods have been developed for using the optical microscope as a quantitative or analytical tool.

Sections in this booklet look at topics that include:
* Resolution and magnification
* Types of optical microscopes
* Illumination of the object
* Dark ground, phase contrast microscopy and fluorescence microscopy
* The polarizing microscope
* The interference microscope
* Automatic methods of image analysis
* Practical notes on the use of the optical microscope

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