This animation shows the major stages of subcloning. This is the process of breaking up fragments of DNA from BAC clones into smaller more manageable pieces for DNA sequencing. This process was very important during the Human Genome Project. Subcloning involves several standard molecular biology techniques including purifying DNA, gel electrophoresis, restriction digests and ligation. 

This resource is part of the Post 16 genetics and genomics collection of resources.

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