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Biotechnology practical archive
A cluster of practical activities for students to explore biotechnology involving viability of yeast, fruit juice production, milk products, fungal inhibition and microbial growth curves.
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Fungal Inhibition
In this experiment students grow yeast on agar plates and look at the effect on growth of some common anti-fungal treatments, which are available to treat conditions such athlete's foot and thrush. Antifungal products may be bought from a local pharmacist, for example, Canistan, Daktarin, Diflucan. Several...
Determining Doubling Time
As the number of cells in a microbial culture increases, turbidity increases. In this experiment students calculate doubling time and growth rate constant using absorbance as the measure of growth. Turbidity is caused by suspended cells in the growth medium scattering light, and may be measured using a colorimeter...
Determining Dry Weight
This experiment is a continuation of Determining Doubling Time. Students create a standard curve of absorbance against yeast concentration, and use this to determine the concentration of yeast produced over time.
The standard curve (dry mass, grams per litre) is generated from known concentrations of dried...
Relative Humidity and Botrytis Infection
From Science & Plants for Schools (SAPS), this practical activity was developed for Scottish Highers specifications, but the protocol can equally well be used for other specifications.
Students incubate...