Lab techniques in mammalian cell biology HC197 / F02

Start date
11 Jul 16
Duration
3 Days
Location
University College London, Gower Street, London WC1E 6BT View on Google Map

Summary

This is a laboratory-based course which provides a framework for further research and experiments. Prominent researchers from UCL will lecture on the theoretical background to the course. You will gain hands-on experience in a range of laboratory techniques. A dedicated team of tutors and technicians will be on hand to guide you through the lab practicals, at your own pace. This course is of interest to researchers in all fields of life sciences and medicine.

The practicals will offer hands-on experience in:

  • good cell culture practice, including safety procedures, control of facilities, equipment, reagents
  • qualitative characteristics of mammalian cell cultures: cell counting and analysis
  • cryopreservation and cell banking
  • sterility and microbial contamination tests
  • maintenance and manipulation of stem cells, primary and established cell lines under various experimental conditions (mitogenic stimulation, exposure to cellular stresses and major signal transduction inhibitors)
  • transient transfection of mammalian cells, expressing wild type and mutant proteins
  • immunofluroescent analysis of subcellular localisation of transiently expressed proteins
  • quantitative analysis of immunofluorescence images by computer software
  • assess the viability and proliferation of cells grown under various experimental conditions using the MTT Cell Proliferation Assay
  • discussion and trouble-shooting

Course lectures will focus on the following topics:

  • whole tissue/cell techniques (immunofluorescence and immunoelectron microscopy, subcellualr fractionation, FACS analysis etc)
  • the control of cellular metabolism and growth by signalling pathways
  • interactions between cells and their environments: general principles in cell signalling and signal transduction
  • regulation of cell survival and proliferation in mammalian cells
  • cell biology underlying vertebrate limb regeneration
  • stem cell biology in health and disease
  • human pluripotent stem cells in culture

This course is recommended for technical staff in biological sciences.

For more information, please visit: www.ucl.ac.uk/cell-biol-cpd

Outcomes

 

Sessions

DateTimeLocation
Lab techniques in mammalian cell biology11 July 201609:00-17:00University College London

Notes

All prices exclude VAT which will be added at the standard rate.

All of our courses will contribute to your evidence base for professional registration https://www.heated.ac.uk/professional-registration/ Just check against your competency measures to make sure you use our training and development in your evidence records.

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Additional information

For courses at the National STEM Learning Centre in York, fees include meals and accommodation for the duration of the course. 

 

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